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. 2017 Oct 19;6(12):2160–2172. doi: 10.1002/sctm.17-0133

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© 2017 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Ectopic bone formation by BCP biomaterial with MSC in the subcutis of nude mice after 8 weeks. (A): Masson trichrome staining on sections through implants with BCP (gray) and MSC from three bone marrow donors (BMSC) and four adipose tissue donors (ATSC), or a mixture of both. Newly formed bone is evidenced in green. Scale bars: 1 mm for images (40 μm for image inserts). The bone incidence score on each group represents the number of implants with newly formed ectopic bone over the total number of implants in that group. (B): Histomorphometry quantification of bone and (C) of mature bone marrow was performed on four sections of every implant. * indicates statistical differences between groups (p < .05). Data are presented as mean ± SE of the mean. Abbreviations: ATSC, adipose tissue‐derived stem cells; BCP, biphasic calcium phosphate; BMSC, bone marrow‐derived stem cells.