Table 1.
Morphometric parameters of lungs. Means with standard errors are presented for normoxic (NormO2) and hyperoxic (HypO2) lungs before (D5) and after (D28, D56) the injection of (PBS) or cells (fresh or cultured for 7 or 77 days)
| Day of test | Cells/PBS | MLI | Alveoli | Tissue Area (*102) | Sec Septa per tissue (×10−1) | Blood vessels | Blood Vessels per tissue | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NormO2 | HypO2 | NormO2 | HypO2 | NormO2 | HypO2 | NormO2 | HypO2 | NormO2 | HypO2 | NormO2 | HypO2 | ||
| D5 | None | 45.0^ ± 2.7 | 57.1** ±2.2 | 16.2^ ± 0.2 | 13.7 ± 0.3 | 16.4^ ±1.2 | 14.5* ±0.8 | 37.7^ ± 2.8 | 26.0** ± 1.7 | 14.6^ ± 0.6 | 12.4* ± 0.5 | 88.6 ± 5.4 | 85.8 ± 6.2 |
| D28 | PBS | 26.7^ ± 0.9 | 32.0** ± 0.9 | 21.8^ ± 0.8 | 19.9** ± 0.4 | 19.5 ± 0.9 | 19.2 ± 0.8 | 25.6^ ± 4.3 | 36.5** ± 2.9 | 19.3 ± 1.1 | 21.5 ± 1.1 | 114.3 ± 3.5 | 116.5 ±0.7 |
| Fresh | 28.4^ ± 0.5 | 29.8^** ± 1.0 | 21.5^ ± 0.6 | 19.9** ± 0.5 | 20.3 ± 0.6 | 18.7 ± 0.5 | 27.8^ ± 4.3 | 30.2^ ± 2.6 | 20.5 ± 0.9 | 20.4 ± 1.1 | 111.2 ± 4.9 | 119.6 ±3.0 | |
| C7 | 27.0^ ± 0.6 | 31.1** ± 0.6 | 21.2 ± 03 | 20.0** ± 0.4 | 20.1 ± 1.2 | 20.9^ ± 0.6 | 23.1^ ± 2.7 | 26.5^ ± 2.1 | 24.6** ± 0.5 | 25.2**^ ± 1.0 | 131.9* ± 3.7 | 108.0 ± 3.3 | |
| C77 | 29.5^** ± 1.0 | 32.3** ± 1.1 | 20.4 ± 0.5 | 20.0* ± 0.6 | 18.3 ± 0.7 | 18.1 ± 0.9 | 34.5** ± 5.6 | 44.4**^ ± 11.7 | 16.4^ ± 2.1 | 19.2 ± .2.3 | 108.0^ ± 22.2 | 118.3 ± 15.4 | |
| D56 | PBS | 30.4^ ± 0.6 | 32.8** ± 1.3 | 20.4 ± 0.2 | 19.6 ± 0.6 | 18.4 ± 0.7 | 17.5 ± 0.6 | 28.3 ± 2.9 | 31.4 ± 1.3 | 22.2^ ± 0.5 | 17.8** ± 1.1 | 108.4 ± 2.8 | 113.1 ± 4.9 |
| Fresh | 32.6** ± 1.9 | 30.1^ ± 1.1 | 19.7 ± 0.9 | 20.3 ± 0.8 | 17.4 ± 1.5 | 18.0 ± 0.8 | 36.3 ± 2.2 | 31.1 ± 3.0 | 20.4 ± 1.9 | 20.0 ± 1.0 | 99.5 ±4.5 | 121.2 ± 4.4 | |
| C7 | 30.9^ ± 1.5 | 32.8* ± 2.6 | 20.0 ± 0.5 | 18.7 ± 0.9 | 17.8 ± 1.3 | 17.4 ± 1.4 | 30.0 ± 3.6 | 34.0 ± 3.7 | 19.1 ± 1.0 | 19.1 ± 1.0 | 112.8 ± 7.0 | 99.7 ± 3.3 | |
| C77 | 31.3 ± 0.8 | 33.0** ± 0.8 | 20.5 ± 1.1 | 18.9 ± 1.0 | 17.7 ± 0.6 | 17.5 ± 0.7 | 35.1 ± 0.8 | 43.1**^ ± 4.9 | 19.4 ± 0.9 | 19.8 ± 1.3 | 110.2 ± 6.1 | 114.5 ± 10.1 | |
Significant differences to respective normoxic controls (dark gray cells) are marked with two asterisks (**, p < .01, n = 4–5) or one asterisk (*, p < .05, n = 4–5). Significant differences to respective hyperoxic controls (light gray cells) are marked with circumflex accent (^, p < .05, n = 4–5).
Abbreviations: alveoli, number of alveoli was counted per 3.63 mm line (vertical and horizontal) drawn across an image of each lung sections, and is presented in airspaces per 1 mm; blood vessels, number of elastin‐positive blood vessels per 0.564 mm2 of lung image; blood vessels per tissue, number of these vessels by 1 mm2 of tissue area; MLI, mean linear intercept (in µm); sec septa, number of secondary septa per 1 mm2 of lung tissue area; tissue area, lung tissue area in mm2 per 0.564 mm2 of lung image.