Table 1.
A summary of top-ranked genes that are differentially regulated by gRNA 9-1 and that suppressed αSyn toxicity in yeast when overexpressed. A complete list of genes differentially modulated by gRNA 9-1 is provided in Table S2.
| Yeast Gene | Human Homologs | Log2(fold change) |
Survival Score |
Fluorescent Foci Score |
Biological Function |
|---|---|---|---|---|---|
| SNO4/HSP34 | DJ-1/PARK7 | 2.035 | 6 | 7 | Chaperone and cysteine protease |
|
| |||||
| HSP32 | DJ-1/PARK7 | −9.593 | 6 | 7 | Chaperone and cysteine protease |
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| |||||
| HSP42 | HSPB1, HSPB3, HSPB6, HSPB7, HSPB8, HSPB9 | 1.434 | 5 | 6 | Chaperone |
|
| |||||
| SIS1 | DNAJB1-B9 | 1.154 | 6 | 3 | Chaperone |
|
| |||||
| GGA1 | GGA1, GGA2, GGA3 | 1.241 | 6 | 6 | ER to Golgi vesicular trafficking |
|
| |||||
| SRN2 | 1.031 | 6 | 4 | Ubiquitin-dependent protein sorting | |
|
| |||||
| SAF1 | ALS2, RCC1 | 1.180 | 6 | 4 | Proteasome-dependent degradation |
|
| |||||
| TRX1 | TXN, TXNDC2, TXNDC8 | 1.072 | 6 | 5 | Thioredoxin |
|
| |||||
| TIM9 | TIMM9 | 3.846 | 6 | 5 | Mitochondrial intermembrane protein |
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| |||||
| OXR1 | OXR1, NCOA7, TLDC2 | 1.003 | 5 | 3 | Oxidative damage resistance |
|
| |||||
| STF2 | SERBP1, HABP4 | 2.004 | 6 | 3 | mRNA stabilization |
|
| |||||
| gRNA 9-1 | 6 | 10 | |||
| UBP3 | 6 | 7 | |||
| Vector | 1 | 1 | |||