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. Author manuscript; available in PMC: 2017 Nov 26.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Nov 15;430(1):289–293. doi: 10.1016/j.bbrc.2012.10.135

Fig. 3.

Fig. 3

Basal p21 protein levels are controlled by the PIRH2-p53 pathway. (A) 104-R cells were transfected with either control siRNA (siCtrl) or two different PIRH2 siRNAs (siPIRH2 #1 and #2) for 48 h. Expression levels of p21, p53, and actin were analyzed by immunoblotting using corresponding antibodies. (B) 104-R cells were transfected with siCtrl, siPIRH2, or p53 siRNA (siP53). Expression levels of p21 and p53 were analyzed by immunoblotting, as described in (A).