Fig. 2.

The linker region 2172–2185 of Piezo1 that connects the central pore and the peripheral propeller-structure is required for SERCA2 interaction. a, Topological and structural illumination of the C-terminal regions of Piezo1 (1960–2547) including the specified featured domains (adopted from PDB 4RAX for the atomic CED structure and 3JAC for the rest of the structure as alanine model²⁷). The residues of the linker region 2172–2185 are shown. The symbols of + and – indicate the relative strength of the interaction. b, d, f, Western blots showing GST pull-down of the co-expressed Flag-SERCA2 proteins using the indicated GST-fused Piezo1 fragment proteins or mutants (repeated 3 times). h, Western blots showing Piezo1-GST pull-down of co-expressed Flag-SERCA2 in the presence of the indicated conditions. The amino acid composition of the linker-peptide and the scrambled peptide are shown in the right (repeated 3 times). c, e, g, i, Scatter plot of the interacting SERCA2 level shown in b, d, f, h, respectively (normalized to the samples represented by the red bars). One-way ANOVA with multiple comparison test. Data shown as mean ± s.e.m. ^** p < 0.01, ***p < 0.001