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. 2017 Nov 27;8:1786. doi: 10.1038/s41467-017-01690-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Cellular expression, localization, and regulation of hHDAC6 during human megakaryopoiesis. a Relative HDAC6 mRNA level expression to HPRT mRNA at different days of MK maturation evaluated by qRT-PCR. b, c Quantification of HDAC6 and acetylated tubulin (Ac-Tubulin) protein levels by western blotting during MK differentiation from two independent experiments. HSC70 is used as a loading control. d Confocal analysis of HDAC6 (red), actin (green), and ac-Tubulin (cyan) localization at different stages of megakaryopoiesis (D9 MK in the upper panel and PPT in the lower one). e The HDAC6 promoter is regulated by RUNX1 and FLI1 in human MKs. Data are presented as fold enrichment by dividing the % ChIP by % IgG. f qRT-PCR analysis of RUNX1 and HDAC6 mRNA levels after RUNX1 knockdown. Error bars in a, e, and f are SD, error bars in c are SEM