Fig. 2.

Effects of HDAC6 inhibition on early human megakaryopoiesis. a, b Effect of increasing doses of HDAC6 inhibitors on the level of Ac-Tubulin and Ac-Histone H3 compared to levels of total Tubulin and Histone H3. a Western blot analysis and b quantification. c–e Effect of increasing doses of HDAC6 inhibitors on c compartment of CFU-MK, d cellular proliferation, and e CD41⁺ CD42⁺ population. f qRT-PCR analysis of HDAC6, HDAC1, HDAC2, and HDAC3 mRNA levels after HDAC6 knockdown with both sh1 and sh2. g Western blot analysis of HDAC6 and Ac-Tubulin in sh HDAC6-transduced MKs and h quantification from two independent experiments. i Mean ploidy value of untreated and Tubastatin- or ACY1215-treated MKs. Results are representative of three independent experiments. Unpaired Student’s t test, *p < 0.05; **p < 0.001; ***p < 0.0001. Error bars in f are SD, all other error bars are SEM