Fig. 7.

NMDAR-Ab from Healthy + subjects and PSY + patients do not affect NMDAR-mediated Ca²⁺ transients in spines of hippocampal neurons. a Representative time-lapse images of a spontaneous NMDAR-mediated Ca²⁺ transient in basal condition (in the presence of nifedipine 5 µM and bicuculline 5 µM). Scale bar, 2 µm. Lower panel, representative examples of NMDAR-mediated Ca²⁺ transients recorded in spines (expressed as ΔF/F ratio) exposed to purified IgG from Healthy −, Healthy +, or PSY + individuals. Note that all events were abolished by the NMDAR-competitive antagonist D-AP5 (50 µM). b Normalized frequency (ratio of Ca²⁺ transients frequency post-application of NMDAR-Ab relative to the baseline acquisition) of spontaneous NMDAR-mediated Ca²⁺ transients in control condition with no IgG (n = 38 spines, N = 4 neurons) or in the presence of Healthy − purified IgG (n = 38, N = 5), Healthy + NMDAR-Ab (n = 68, N = 10), or PSY + NMDAR-Ab (n = 131, N = 15). Data are expressed as mean ± SEM. One out of one Healthy − , three out of three Healthy + , and four out of nine PSY + were used and pooled for comparisons. P > 0.05, Krukal–Wallis test