Figure 2.

Western blotting of cleaved high-molecular weight kininogen. During contact system activation, single-chain HK is converted into a two-chain disulfide-linked product. In the example shown here, plasma samples (activated in vitro with kaolin) were separated on a 4–12% gradient gel under reduced conditions. This separates the two chains. HK was subsequently detected with a polyclonal antibody that preferentially recognizes HK light chain. Molecular weights are shown on the left (in kDa).