Figure 1.

Platelet aggregation, thrombin generation, and fibrinolysis in LZR and MSZR rats. (A) Mean maximum aggregation in washed platelets in response to collagen (5 μg/ml) and in (B) platelet-rich plasma (PRP) in response to ADP (5 μM), with the platelet count adjusted to 200 × 10⁹ platelets/l. (C) Calibrated automated thrombinography (CAT) in rat plasma. Mean thrombin generation curves in platelet free plasma (PFP) triggered by 5 pM tissue factor in LZR and MSZR at 25 and 80 weeks of age. (D) Endogenous thrombin potential (ETP) in PFP and PRP of 25 and 80 week-old LZR and MSZR, expressed as ratios of values for 25 week-old LZR. (E) Ultrastructure of fibrin fibers was visualized by scanning electron microscopy. Pictures were made at 10,000 × magnification. (F,G) Fiber thickness and fiber density of fibrin clot in LZR and MSZR. (H) ELISA results of PAI-1 measured in PFP (n = 17–19). (I) Representative curves of fibrinolytic tests in PFP in LZR and MSZR. (J,K) Half-lysis time and maximal lysis speed of fibrinolytic tests in LZR and MSZR. Results are mean ± standard error of the mean (n = 7–11). ^*p < 0.05 vs. LZR at the same age; ^#p < 0.05 vs. 25 week-old rats in the same strain.