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. 2017 Nov 22;5:97. doi: 10.3389/fcell.2017.00097

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Copyright © 2017 Rusin, Adamo and Kettenbach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mitotic CK2 AP-MS analysis. (A) Scheme depiction of tetrameric CK2 complex found in cells. (B) Scheme for identification of mitotic CK2 interactors. Cells were transfected with different FLAG-tagged CK2 subunits or control and mitotically arrested. Cells were collected, lysed, and FLAG-tagged proteins were purified, TCA precipitated, and analyzed by LC-MS/MS. (C) Volcano plots of the log₂ difference in intensity between sample and control against the negative log₁₀ of the p-value of the replicate fold change for each of the three purifications performed.