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. 2017 Nov 22;8:2311. doi: 10.3389/fmicb.2017.02311

FIGURE 4.

FIGURE 4

Attachment assays with PAO1 mutants. (A) Attachment of P. aeruginosa PAO1 wildtype (WT) or PAO1 mutant strains during overexpression of PA3177 was evaluated by crystal violet staining. Bacteria were incubated in 96-well microtiter plates for 2 h at 37°C prior to biomass quantification. For recombinant gene expression, L was supplemented with 0.1% (w/v) arabinose (+). Cultures without arabinose (-) served as negative controls. (B) Attachment of P. aeruginosa PAO1 WT or PAO1 deletion mutants during NaClO treatment (2 μg/ml in BM2, 2 h) was evaluated by crystal violet staining. All experiments were carried out in triplicate, each with six wells per strain and condition (n = 18). Statistical significance was evaluated by the Mann–Whitney test (∗∗∗p ≤ 0.001, ∗∗p ≤ 0.01, n.s., not significant). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers.