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. 2017 Sep 18;292(47):19198–19208. doi: 10.1074/jbc.M116.773093

Figure 5.

Figure 5.

Inhibition of Rab7 GTPase impaired lal−/− EC permeability and migration and decreased ROS overproduction. A, EC migration after Rab7 GTPase siRNA knockdown was assessed by an in vitro wound healing assay in the presence of mitomycin C. C, control. B, MDSC transendothelial migration was determined after Rab7 GTPase siRNA knockdown in ECs for 48 h. CMFDA-labeled bone marrow–derived MDSCs were added to the siRNA-transfected EC monolayers. Four hours later, MDSCs in the lower chamber were counted. C, EC proliferation after Rab7 GTPase siRNA knockdown. D, statistical analysis of mean fluorescent intensity (MFI) of the ROS level in ECs after Rab7 GTPase siRNA knockdown by flow cytometry. For all experiments, scrambled siRNA transfection served as a control, and data are expressed as mean ± S.D. n = 4∼5. *, p < 0.05; **p < 0.01.