Figure 7.

Inhibition of Rab7 GTPase increased tube formation and tumor binding to lal^−/− ECs. A, flow cytometry analysis of VEGFR2 expression in ECs after Rab7 GTPase siRNA knockdown. C, control. B, EC in vitro Matrigel tube formation was assessed after Rab7 GTPase siRNA knockdown. Statistical analysis of cumulative tube lengths 6 h after EC seeding on Matrigel is shown. C, CMFDA-labeled tumor cell (B16 melanoma or LLC cells, 1 × 10⁴) adhesion to ECs after Rab7 GTPase siRNA knockdown. Five fields in each well were quantified using a fluorescence microscope. D, flow cytometry analysis of expression of E-selectin and P-selectin in ECs after Rab7 GTPase siRNA knockdown. In all experiments, scrambled siRNA transfection served as a control, and data are expressed as mean ± S.D. n = 4∼5. *, p < 0.05; **, p < 0.01.