Figure 5.

Typical ion current recordings through a single EcChiP channel at different voltages. Ion current fluctuations were monitored for 120 s at applied potentials of ±100 mV with sugars on either the cis or the trans side in 1 m KCl in 20 mm HEPES, pH 7.4. Here, only current traces for 1,000 ms at cis are presented. A–D, ion current fluctuations in a single EcChiP channel in the presence of 5 μm chitohexaose at +25, +50, +100, and +150 mV, respectively. E, association rates on cis with potentials ranging from ±25 to ±199 mV. F, dissociation rates on cis with potentials ranging from ±25 to ±199 mV. G, association rates on trans with potentials ranging from ±25 to ±199 mV in the presence of 5 μm chitohexaose. H, dissociation rates on trans with potentials ranging from ±25 to ±199 mV in the presence of 5 μm chitohexaose.