Table 4.
Measurements for TFIIE-binding dynamics at select promoters
| TFIIE | kxla | τxlb | ka·CTFc | kdd | Ssate | Kf | t½ g | θbh |
|---|---|---|---|---|---|---|---|---|
| 1/mol s | s | 1/s | 1/s | mol | ||||
| Full model fit | ||||||||
| ACT1 | 4.80E-03 (+1.3, −0.22) | 80.2 (+992.3, −16.85) | 3.5 (+9.4, −2.7) E-04 | 2.0 (+7.2, −2.2) E-03 | 3.11 (+3.21, −2.94) | 7.80 (+14, −6.2) E-05 | 346.6 (+523.9, −156.8) | 0.15 (+0.48, −0.01) |
| XL-limited fit | ||||||||
| URA1 | 1.96 (+1.0, −0.69) E-03 | 206.5 (+76.52, −52.94) | NAi | NA | 2.71 (+2.71, −0.48) | 7.17 (+18.0, −5.5) E-06 | NA | 0.66 (+0.15, −0.12) |
| LOS1 | 4.3 (+5.4, −2.5) E-03 | 90.1 (+95.4, −44.2) | NA | NA | 0.29 (+0.08, −0.05) | 4.14 (+2.4, −1.0) E-05 | NA | 0.31 (+0.14, −0.1) |
a Formaldehyde cross-linking rate is shown.
b Cross-linking time is shown.
c On-rate of transcription factor X nuclear concentration of factor is shown.
d Off-rate of transcription factor is shown.
e ChIP signal at saturation is shown.
f Dissociation constant, kd/ka is shown.
g Residence time of TF binding is shown.
h Occupancy is shown.
i NA means not applicable.