Figure 6.
Effect of fractional acylation of WT apo-SOD1 with weakly charged groups (ΔZformal ≤ −2) on SOD1 fibrillization into ThT-positive species. a and b, replicate ThT fluorescence assays of unmodified (a) and singly acylated (b) WT apo-SOD1. Replicates that resulted in zero fluorescence (denoted ThT(−)) are also shown. c, plots of average ThT fluorescence for all unmodified and acylated WT apo-SOD1 (averaged from a and b). Replicates that resulted in zero fluorescence (i.e. ThT(−)) were included in average plots. d, SDS-PAGE of all apo-SOD1 solutions before starting (left gel) and after termination of ThT assay (right gel). The TEM micrographs for all modified and unmodified SOD1 proteins are shown in supplemental Fig. S11.