Figure 7.
Effect of acylation on the probability of SOD1 aggregation studied with Kaplan–Meier estimators. Kaplan–Meier plots of SOD1 aggregation in microplate wells, extracted from ThT fluorescence data in Figs. 5 and 6. Each down-step represents aggregation of a single well into a ThT-positive species. The red trace in each plot represents acylated protein, and the black trace represents the unacylated control. Values next to each plot indicate the percentage of wells that showed no significant increase in ThT fluorescence over 120 h (i.e. ThT-negative wells). In all plots, p values were calculated with respect to unmodified SOD1 using log rank (Mantel–Cox) algorithm at 95% confidence interval (p < 0.05).