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. 2017 Oct 3;292(47):19366–19380. doi: 10.1074/jbc.M117.805283

Figure 9.

Figure 9.

Seeding properties of unmodified (a–c) and singly pyromellitylated fibrils (d–f) of WT apo-SOD1 after 4-week incubation with organotypic spinal cord cultures from transgenic ALS mice. Representative confocal micrographs of inclusion pathology induced in spinal cord slices from transgenic G85R-SOD1·YFP mice (harvested at ∼7 days old). Fibrils were grown in microplate wells and added in a well-specific manner to organotypic spinal cord as a function of ThT fluorescence: high fluorescence (a and f), low fluorescence (b and e), and zero fluorescence (c and d). The value on the top right of each confocal micrograph represents the percentage of replicate spinal cord slices that showed SOD1 inclusions (n = 5). The plots below the confocal micrographs show ThT fluorescence traces (left) and TEM micrographs (right) corresponding to the particular microplate well/fibril solution that was added to spinal cord.

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