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. 2017 Oct 11;292(47):19441–19457. doi: 10.1074/jbc.M117.815910

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Figure 2. — Purification and identification of GlcNAc-phosphate-undecaprenol in 5005ΔgacL. A, TLC analysis of phospholipids isolated from MGAS5005 and 5005ΔgacL. Phospholipids extracted from bacterial strains were separated by TLC on Silica Gel G in CHCl₃/CH₃OH/H₂O/NH₄OH (65:25:4:1). Position of the novel, alkaline-resistant, and acid-labile phospholipid accumulating in 5005ΔgacL is indicated by the arrow. The results are representative of three separate experiments. B, ESI-MS/MS analysis of the novel phospholipid isolated from 5005ΔgacL. The spectrum is assigned to GlcNAc-phosphate-undecaprenol.