Figure 4.

ELC1 interacts with the MyoA converter subdomain, increasing its affinity for the MyoA neck and stabilizing the myosin lever arm. Schematics shown are colored as previously. a, representative ITC binding isotherm of ELC1 titrated into MyoA (1–831) prebound with MLC1. b, HDX difference map showing reduction in total deuteron incorporation of full-length MyoA, prebound with MLC1, when ELC1 is added. The decreases in amide exchange between conditions are summed over three time points of HDX (3, 30, and 300 s at 23 °C), and the error bars (S.D.) represent independent triplicate. Peptide # corresponds to the centroid amino acid of the peptide from which a data point is obtained. Inset, expanded view of HDX difference map showing order induced in MyoA converter domain (residues ∼710–740) by ELC1 binding. c, representative deuterium incorporation time course for MyoA peptide in the converter subdomain (716–720). Refer to supplemental Table S3 for the full HDX-MS data set relating to this figure.