Table 1.
Summary of ITC thermodynamic parameters
All experiments were performed in triplicate. Thermodynamic parameters were fitted using Origin software (Microcal).
| Light chain | MyoA/light chain | CaCl2 | Molar ratio | Kd | ΔH | −TΔS |
|---|---|---|---|---|---|---|
| nm | kcal/mol | kcal/mol | ||||
| MLC1 | 801–818 | − | 1.05 ± 0.01 | 10.0 ± 1.4 | −26.9 ± 0.2 | 16.0 ± 0.2 |
| 775–818 | − | 1.00 ± 0.01 | 43.1 ± 6.5 | −40.7 ± 0.6 | 30.6 ± 0.6 | |
| ELC1 | 775–818 | − | 0.99 ± 0.03 | 590.6 ± 46.5 | −5.7 ± 0.2 | −3.0 ± 0.1 |
| + | 1.02 ± 0.03 | 82.8 ± 17.7 | −10.4 ± 0.1 | 0.8 ± 0.2 | ||
| 775–818/MLC1 | − | 1.02 ± 0.04 | 71.8 ± 5.0 | −9.9 ± 0.1 | 0.1 ± 0.1 | |
| + | 1.02 ± 0.04 | 11.5 ± 5.2 | −13.2 ± 0.4 | 2.3 ± 0.5 | ||
| FL/MLC1 | − | 1.02 ± 0.01 | 1.9 ± 1.5 | −15.8 ± 0.3 | 3.7 ± 0.8 | |
| ELC1 WT | + | 0.97 ± 0.38 | 2.3 E4 ± 0.9 E4 | 1.3 ± 0.3 | −7.6 ± 0.1 | |
| MgCl2 | 0.34 ± 0.09 | 14.4 E4 ± 2.1 E4 | 9.0 ± 2.5 | −14.3 ± 2.4 | ||
| ELC1 D80A | + | 1.43 ± 0.05 | 1.8 E4 ± 0.3 E4 | 0.9 ± 0.1 | −7.3 ± 0.1 |