Figure 5.

The dNLP-ISWI system can be used to assemble HMGN2-containing chromatin. A, DNA supercoiling analysis of chromatin assembled with HMGN2. Chromatin assembly reactions were performed as described in the legend for Fig. 3, in the absence or presence of purified human HMNG2 (shown in Fig. 4A) at the indicated molar ratios of HMGN2 to nucleosomes. B, partial MNase analysis of HMGN2-containing chromatin assembled as described in A. C, native gel electrophoresis of mono- and dinucleosomes obtained by extensive MNase digestion of chromatin either lacking or containing HMGN2. The chromatin particles were detected by staining of the DNA with SYBR Gold. The positions of mononucleosomes (mono), dinucleosomes (di), mononucleosomes containing one molecule of HMGN2 (mono + 1 HMGN2), and mononucleosomes containing two molecules of HMGN2 (mono + 2 HMGN2) are indicated.