Figure 2.

Purification of NtPDR1. NtPDR1 was solubilized from 800 mg of a microsomal fraction and purified as described under “Experimental procedures.” Twenty microliters of solubilized material (SN), flow-through of the first Ni-NTA column (FT₁), elutions of the first and second Ni-NTA columns (E₁ and E₂), elution of the desalting column (P10), the flow-through and elution of the third column (FT₃ and E₃), and 5 μl of concentrated flow-through 3 were analyzed by SDS-PAGE and Coomassie Blue staining. The star shows at which step the purification tag was cleaved using TEV protease. The arrow indicates the band corresponding to NtPDR1.