Figure 8.

Stimulation of NtPDR1:GUS expression by methyl jasmonate. Leaf discs of N. tabacum plants expressing the GUS reporter gene under the control of the NtPDR1 promoter (25) were incubated with water or with 250 μm methyl jasmonate, and the in situ GUS activity was monitored 24 h later. A, control leaf disk incubated with water. The left panel shows a top view of a leaf disk. GUS expression is limited to trichomes. The right panel shows a trichome in detail. B, leaf disk incubated with 250 μm methyl jasmonate. Expression was observed in the whole leaf tissue. Arrows indicate examples of stained trichomes head cells.