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. 2017 Sep 26;292(47):19503–19520. doi: 10.1074/jbc.M117.791558

Table 1.

Cytotoxicity of K. oxytoca strain MH43-1 culture supernatants from different culture conditions as measured in a cell culture cytotoxicity assay

All cultures were incubated in a shaker at 250 rpm under aerobic conditions for 24 h.

Culture media Temperature Highest dilution with observable cytopathic effects
LB broth (Sigma) 37 °C 2–4×
Brain-heart infusion (Sigma) 37 °C
Todd-Hewitt broth 37 °C
Tryptic soy broth (TSB) 37 °C
TSB 35 °C 16×
TSB 42 °C No cytotoxicity
Tryptone lactose broth (TLB) 35 °C 64×
TLB 42 °C No cytotoxicity
Tryptone glucose broth 35 °C 32–64×