Table 1.
Cytotoxicity of K. oxytoca strain MH43-1 culture supernatants from different culture conditions as measured in a cell culture cytotoxicity assay
All cultures were incubated in a shaker at 250 rpm under aerobic conditions for 24 h.
Culture media | Temperature | Highest dilution with observable cytopathic effects |
---|---|---|
LB broth (Sigma) | 37 °C | 2–4× |
Brain-heart infusion (Sigma) | 37 °C | 4× |
Todd-Hewitt broth | 37 °C | 4× |
Tryptic soy broth (TSB) | 37 °C | 4× |
TSB | 35 °C | 16× |
TSB | 42 °C | No cytotoxicity |
Tryptone lactose broth (TLB) | 35 °C | 64× |
TLB | 42 °C | No cytotoxicity |
Tryptone glucose broth | 35 °C | 32–64× |