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. 2017 Sep 16;292(47):19537–19545. doi: 10.1074/jbc.M117.813469

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 3. — Effect of multiple sequence modifications on simulated integration efficiency and experimental expression is cumulative and nearly independent. A, the simulated integration efficiency of double-loop-swap chimeras (vertical axis) versus the product of the simulated integration efficiencies of the constituent single-loop-swap chimeras (horizontal axis). The guideline with a slope of 0.8 indicates that the effect of loop-swap mutations on simulated integration efficiency is cumulative and largely independent. B, the experimental expression of double-loop-swap chimeras (vertical axis) versus the product of the experimental expression values of the constituent single-loop-swap chimeras (horizontal axis). The guideline with a slope of 0.7 indicates that the effect of loop-swap mutations on experimental expression is also cumulative and largely independent. Error bars, S.E.