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. 2017 Jul 31;36(47):6542–6554. doi: 10.1038/onc.2017.262

Figure 6.

Figure 6

Effect of increased ROS1 on MAPK, PI3K-AKT signaling pathways, cell proliferation, migration, and invasion. (a) Western blotting was carried out with lysates of OC3 and OC3-IV2 cells using anti-ROS1 and anti-pROS1(Y2274). The value for pROS1(Y2274) was normalized to total ROS1. (b and c) Relative protein levels of pAKT(S473), AKT, pERK1/2, ERK1/2, pSTAT3(Y705), and STAT3 in OC3, OC3-IV2, OC3-IV2-Scr, and OC3-IV2-shROS1#1 cells as determined with Western blotting. Levels of pERK1/2, pAKT(S473), and pSTAT3(Y705) were normalized to those of total ERK1/2, AKT, and STAT3, respectively, and then to OC3 and OC3-IV2-Scr cells. (d) pAKT(S473), AKT, pERK1/2, and ERK1/2 levels in lysates from OC3 and OC3-IV2 cells treated with 20 μM U0126 or LY294002 for 24 h. (e and f) Relative proliferation and migration of OC3 and OC3-IV2 cells treated with 20 μM U0126 or LY294002 for 24 or 48 h were assessed using MTT and wound-healing assays, respectively. *, #Compared with DMSO treatment. (g) Migration and invasion of OC3-IV2 cells treated with 20 μM U0126 or LY294002 for 26 h were assessed with the Boyden chamber assay. Data from at least three independent experiments are presented as mean±SEM (*, #P<0.05).