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. 2017 Jul 31;36(47):6518–6530. doi: 10.1038/onc.2017.257

Figure 4.

Figure 4

miR-377-3p, miR-1343-3p and miR-4269 are tumor-suppressive miRNAs. (a) Overexpression of miR-377-3p, miR-1343-3p and miR-4269 suppressed cell proliferation in a 4-day MTT assay (**P<0.05; **P<0.001; unpaired t-test). (b) Smaller colony size and reduced colony number were observed in miR-377-3p, miR-1343-3p and miR-4269 transfectants compared with negative control (**P<0.001; unpaired t-test). (c) Cell invasion abilities were impaired by ectopic miR-377-3p, miR-1343-3p and miR-4269 expression in MGC-803 and SGC-7901 cells (**P<0.001; unpaired t-test). (d) Cell cycle analysis by flow cytometry revealed that miR-1343-3p and miR-4269 overexpression increased the proportion of G0/G1-phase cells (**P<0.001; unpaired t-test). (e) miR-377-3p and miR-4269 promoted late apoptosis in GC cells (**P<0.001; unpaired t-test). (f) Western blot analysis of cell cycle regulators and apoptosis-related markers, validating the flow cytometry results.