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. 2017 Aug 4;11(12):2754–2766. doi: 10.1038/ismej.2017.125

Figure 3.

Figure 3

Testing H2S Production by bacteria in LB. Bacteria were inoculated in 2 ml of LB in 15-ml tubes with the lead-acetate filter paper fixed at the top of the headspace. (Row A) A1, E. coli BL21(DE3) (Ec); A2, Ec(Pasqr1); A3, Ec(Pasqr2); A4, Ec(Papdo); A5, Ec(Papdo-Pasqr2); A6, Ec(Cppdo2-Cpsqr). (Row B) B1, P. aeruginosa PAO1 (Pa); B2, PaΔsqr1Δsqr2; B3, PaΔpdo; B4, PaΔsqr1Δsqr2Δpdo (Pa3K), B5: Pa3K(Papdo-Pasqr2). (Row C) C1, C. pinatubonensis JMP134 (Cp); C2, CpΔsqr; C3, CpΔpdo2Δsqr (Cp2K); C4, Cp2K(Cppdo2-Cpsqr). (Row D) NaHS standards in LB: D1, 100 μm; D2, 50 μm; D3, 25 μm; D4, 10 μm; D5, 5 μm; D6, 0 μm. E. coli and P. aeruginosa were incubated at 37 °C, and C. pinatubonensis was incubated at 30 °C for 18 h.