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. 2017 Nov 22;8:2302. doi: 10.3389/fmicb.2017.02302

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Copyright © 2017 Yoshida, Murata, Ashio, Narita, Watanabe, Masud, Sato, Goshima and Kimura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction of recombinant EBV featuring a mutation in the LMP1 promoter. (A) Schematic arrangement of recombination of the EBV genome using tandemly arranged neomycin-resistance and streptomycin-sensitivity genes (Neo/st). The B95-8 ED-L1 LMP1 promoter (–360 to –11) was first replaced with the Neo/st cassette, which was then replaced with mutated sequences (ringed X) to construct mutant EBV-BACs. TR, terminal repeat; TR-L1p, TR leftward promoter 1 (distal LMP1 promoter); ED-L1p, EcoRI-Dhet fragment leftward promoter 1 (proximal LMP1 promoter). (B) Electrophoresis of recombinant virus genomes. Recombinant EBV genomes were digested with BamHI or EcoRI and resolved on an agarose gel.