Skip to main content
. 2017 Nov 22;8:2302. doi: 10.3389/fmicb.2017.02302

FIGURE 3.

FIGURE 3

The mEbox/Ikaros mutation affected LMP1 expression in Akata cells but not in HEK293 cells. (A–D) EBV-BAC DNAs prepared as shown in Figure 2 were introduced into HEK293 cells, and the LMP1 levels were measured by qRT-PCR (A,B) and immunoblotting (C,D) using anti-LMP1, -EBNA1, and -Tubulin antibodies. The relative levels of mRNA encoding LMP1 were normalized to the EBV copy numbers. (E,F) Infectious recombinant viruses (WT or mutants) were produced from the HEK293 cell lines described above, and equivalent numbers of viruses were used to infect EBV-negative Akata(-) cells. Cell RNA was harvested after 2 days and subjected to qRT-PCR to determine the levels of LMP1. Relative mRNA levels of LMP1 are shown after normalization to the EBV copy numbers. Each bar represents the mean and standard deviation of three independent infections. Student’s t-test was performed. ∗∗∗∗∗p < 0.002.