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. 2017 Nov 22;8:2315. doi: 10.3389/fmicb.2017.02315

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Copyright © 2017 Huo, Rong, Jian, Xu, Li and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of NaCl and metal ions on the activity of E69. (A) The effect of NaCl on the activity of E69 was determined using p-NP butyrate as the substrate. The value obtained without added NaCl was taken as 100%. (B) The effects of metal ions and EDTA on the activity of E69 were determined using p-NP butyrate as the substrate. All of the tests were performed at 40°C and pH 10.0. The value obtained without metal ions was taken as 100%. Data are presented as mean ± SD (n = 3). ^∗p < 0.05, representing a significant difference from the 100% values (Student’s t-test).