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. Author manuscript; available in PMC: 2017 Nov 27.
Published in final edited form as: J Am Chem Soc. 2015 Apr 2;137(14):4598–4601. doi: 10.1021/ja511298n

Figure 2.

Figure 2

CTB's function is enhanced when conjugated on NanoScript. (a) The CTB molecule is modified with a thiol-PEG chain and functionalized on NanoScript. The remaining components on NanoScript include the AD, which recruits endogenous factors to initiate transcription, and the DBD (refer to Figure S1 for structure details), which targets and binds to the target Sox9 sequence on DNA (b) The HAT activity of this NanoScript-Sox9 construct was shown to increase across three time points (days 1–3). Controls with only the AD (without CTB), only CTB (without AD), or unconjugated CTB in media showed comparatively less HAT activity. All conditions and controls were transfected with 1 nM of the constructs. The increase of HAT activity for all samples was determined by normalizing to the untreated control. Standard error is from three independent trials.