Figure 4.

Identification of a putative new glutelin with a preferential embryo accumulation. (A) Boxplots of protein abundances (log₂ transformed) for the 10 glutelins that are commonly detected in the endosperm (A0, dark red) and the embryo (E0, blue). The Glu-X protein corresponds to a newly discovered glutelin which is the only glutelin more abundant in the embryo although not significantly. Significantly different means are indicated according to the z-score analysis with p-values inferior to 0.01 (^**) or 0.05 (^*). (B) Close-up view of the conserved cleavage site of glutelin precursor into acidic and basic subunits. The GluC-1 protein sequence does not exibit the characteristic NG motif. (C) Phylogenetic tree built from the 12 glutelin protein sequences together with the newly putative Glutelin, namely Glu-X (neighbor joining, BLOSUM62 matrix)