Figure 5.
In vitro DOX delivery and analysis. (a) MOS-J osteosarcoma normalized mitochondrial activity or viability after exposure to various concentrations of DOX. IC50 of DOX was observed to be at concentrations above 10 µg/mL. (b) Live/Dead staining of MOS-Js revealed a greater number of dead cells when exposed to free DOX as compared to DOX-loaded nanogels and nanocomposites (Scale bar 100µm). (c) MTT assay of MOS-Js exposed to various experimental groups. DOX-loaded nanogels significantly increased (***p<0.001) cell viability in comparison to free DOX. Additionally, incorporation of DOX-loaded nanogels in the GelMA matrix further increased cell viability. (d) MC3T3s were also exposed to free DOX and DOX-loaded nanogels and a similar response in cell viability was observed (Scale bar 100µm). (e) comparison of MC3T3 viability of various experimental groups normalized to untreated control. A significant increase (***p<0.001) in cell viability was observed in the DOX-loaded nanogels.