Table 1.
Gene | Accession number | Sequence | Melting temperature (°C) |
---|---|---|---|
GAPDH | NM_002046 | Forward: 5′-GAACATCATCCCTGCCTCTACTG-3′ Reverse: 5′-TCCGACGCCTGCTTCACC-3′ |
58.2 58.8 |
| |||
COL1A1 | NM_000088 | Forward: 5′-CAGGGCGACAGAGGCATAAAG-3′ Reverse: 5′-CCAGCCATTGATACAGGTAGC-3′ |
58.3 58.3 |
| |||
OCN | NM_000711 | Forward: 5′-GCAGAGTCCAGCAAAGGTG-3′ Reverse: 5′-CCAGCCATTGATACAGGTAGC-3′ |
55.5 55.7 |