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. 2017 Nov 13;2017:7124817. doi: 10.1155/2017/7124817

Table 1.

Primer information for real-time PCR.

Gene Accession number Sequence Melting temperature (°C)
GAPDH NM_002046 Forward: 5′-GAACATCATCCCTGCCTCTACTG-3′ 
Reverse: 5′-TCCGACGCCTGCTTCACC-3′
58.2
58.8

COL1A1 NM_000088 Forward: 5′-CAGGGCGACAGAGGCATAAAG-3′ 
Reverse: 5′-CCAGCCATTGATACAGGTAGC-3′
58.3
58.3

OCN NM_000711 Forward: 5′-GCAGAGTCCAGCAAAGGTG-3′ 
Reverse: 5′-CCAGCCATTGATACAGGTAGC-3′
55.5
55.7