Figure 4.

Normalized volume of isolated rat hepatocytes (A) and Brx-142 cells (B) when they are in equilibration with a series of hypertonic solutions. The function $V/V_0$= $f\left({\pi }_0/\pi \right)$ is described by the Boyle-van’t Hoff relation to determine the osmotically inactive volume fraction. (A: $V/V_0$=0.6587 ${\pi }_0/\pi$+0.4061 ( $R^2$=0.99) and B: $V/V_0$=0.6103 ${\pi }_0/\pi$+0.3611 ( $R^2$=0.9853))