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. Author manuscript; available in PMC: 2017 Nov 27.
Published in final edited form as: Int J Biochem Cell Biol. 2016 Aug 30;79:139–150. doi: 10.1016/j.biocel.2016.08.023

Fig. 5. VEGF receptors are involved in leptin-induced Notch mRNA in endothelial cells.

Fig. 5

A. Leptin dose-dependent induction of Notch mRNA in PAEC wild type; B. PAEC-VEGFR-1 and C. PAEC-VEGFR-2. Quantitative results from real-time PCR of Notch mRNA expression in PAEC wild type and PAEC transfected with VEGFR-1 and VEGFR-2. Cells were cultured in medium containing leptin (0.6,1.2, and 6.2 nM) for 24 h. Cell lysates were used to determine Notch mRNA expression after treatment. RNA expression was calculated by normalizing values to GAPDH mRNA. Relative mRNA expression was calculated to basal. Data is presented as an average ± s.d. from three independent experiments. * p<0.05 when compared to basal.