Table I.
| A. TCRβ rearrangement phenotypes in Jβ1DJβ/DJβ and Jβ1DJβ/ω αβ T cell hybridomas1 | |||
|---|---|---|---|
| Genotype | Total | VβDJβ/DJβ | VβDJβ/VβDJβ |
| Jβ1DJβ/DJβ | 88 | 55 (62.5%) | 33 (37.5%) |
| Jβ1DJβ/ω | 247 | 144 (58.0%) | 103 (42.0%) |
| B. Frequency of complete VβDJβ exon rearrangements on the DJβ allele versus the ω allele in heterozygous VβDJβ/DJβ Jβ1DJβ/ω αβ T cell hybridomas (144 total)2 | ||
|---|---|---|
| Genotype | Allele | Vβ-to-DJβ |
| Jβ1DJβ/ω | DJβ | 99 (69.0%) |
| ω | 45 (31.0%) | |
1
Results for sections A and B were collected from hybridomas grown from at least three independent fusions. See Materials and Methods for details.
2
VβDJβ rearrangements in Jβ1DJβ/ω mice could be linked to the different alleles using a unique restriction digest pattern introduced in the DJβ targeting. See Materials and Methods and FIGURE 1 for details.