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. 2017 Nov 23;10:5591–5604. doi: 10.2147/OTT.S149632

Figure 2.

Figure 2

Specific expression of transgenes mediated by oncolytic adenoviruses in PAC cells.

Notes: (A) Cell lines were planted in 24-well plates at 1×105 per well and infected with the recombined adenoviruses, AdCEAp-EGFP and Ad5-EGFP, at an MOI of 1 pfu/cell. After 48 hours postinfection, EGFP expression was observed under a fluorescent microscope. Magnification: 100×. (B) Cell lines were planted in 6-well plates at 1×106 per well and infected with the recombined adenoviruses, AdCEAp-miR126/34a and Ad5-miR126/34a, at an MOI of 1 pfu/cell. After 48 hours postinfection, cells were collected and used to detect the expression of miR-126 and miR-34a by qRT-PCR. *P<0.05, **P<0.01, and ***P<0.001 versus the parental cells within the same cell line; ##P<0.01 and ###P<0.001 versus the SW1990 parental cells.

Abbreviations: AdCEAp-miR126/34a, carcinoembryonic antigen promoter-driven oncolytic adenovirus; PAC, pancreatic adenocarcinoma; CEA, carcinoembryonic antigen; MOI, multiplicity of infection; EGFP, enhanced green fluorescent protein; qRT-PCR, quantitative reverse transcription polymerase chain reaction.