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. 2017 Nov 23;10:5605–5619. doi: 10.2147/OTT.S149200

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© 2017 Xie et al. This work is published and licensed by Dove Medical Press Limited

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Analysis of RKIP expression levels in transfected NSCLC cell lines by Western blots.

Notes: (A) Western blots showed RKIP expression levels in stable transfected cell lines and the corresponding control cell lines. β-actin was used as a loading control. (B) Histograms show relative expression levels of RKIP in these cell lines by grayscale analysis. Data are presented as the mean ± SD. *P<0.05 vs A549; ^#P<0.05 vs SK-MES-1. All experiments were performed in triplicate.

Abbreviations: A549, A549 cells without transfection; A-EV, A549 cells transfected with empty vector; A/RKIP-KD, A549 cells transfected with RKIP-knockdown vector; EV, empty vector; KD, knockdown; NSCLC, non-small-cell lung cancer; RKIP, Raf kinase inhibitor protein; SK-MES-1, SK-MES-1 cells without transfection; SK-EV, SK-MES-1 cells transfected with empty vector; SK/RKIP-KD, SK-MES-1 cells transfected with RKIP-knockdown vector.