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. 2017 Nov 23;10:5605–5619. doi: 10.2147/OTT.S149200

Figure 4.

Figure 4

RKIP reduction enhances NSCLC cell migration and invasion abilities.

Notes: (A) A representative result showing the migration and invasion abilities of A/RKIP-KD, SK/RKIP-KD and of their corresponding control cell lines A-EV and SK-EV, depicted in Transwell chambers. The cells were incubated in the upper chamber for 48 h. (B) Histograms show numbers of cells of A/RKIP-KD, SK/RKIP-KD and their corresponding control cell lines A-EV and SK-EV penetrating into the lower chambers. **P<0.01. (C) Histograms show the relative wound closure (present vacant area/original vacant area) of the NSCLC cell lines in wound-healing assays at different time points. Data are presented as the mean ± SD (P<0.05). All experiments were performed in triplicate.

Abbreviations: EV, empty vector; KD, knockdown; NSCLC, non-small-cell lung cancer; RKIP, Raf kinase inhibitor protein; A-EV, A549 cells transfected with empty vector; A/RKIP-KD, A549 cells transfected with RKIP-knockdown vector; SK-EV, SK-MES-1 cells transfected with empty vector; SK/RKIP-KD, SK-MES-1 cells transfected with RKIP-knockdown vector.