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Gradient elution of the UPLC-UV method
| Time (minutes) | Phase A (%) | Phase B (%) | Flow (mL/minutes) |
|---|---|---|---|
| 2.5 | 100 | 0 | 0.6 |
| 4 | 20 | 80 | 0.6 |
| 6 | 20 | 80 | 0.6 |
| 8 | 100 | 0 | 0.3 |
| 9 | 100 | 0 | 0.6 |
| 10 | 100 | 0 | 0.6 |
Note: Phase A was composed of water and phase B of methanol.
Abbreviation: UPLC-UV, ultra-high-performance liquid chromatography.
