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Gradient elution of the LC-MS/MS method
| Time (minutes) | Phase A (%) | Phase B (%) | Flow (mL/minutes) |
|---|---|---|---|
| 0.00 | 20 | 80 | 0.200 |
| 6.00 | 28 | 72 | 0.200 |
| 6.10 | 28 | 72 | 0.300 |
| 7.00 | 20 | 80 | 0.300 |
| 12.00 | 20 | 80 | 0.300 |
Note: Mobile Phase A was composed of a mixture of ammonium acetate 10 mM and 0.1% formic acid v/v, and mobile phase B acetonitrile and 0.1% formic acid v/v.
