Table 2.
Characterization of 21 EST-SSR primers developed in Sinowilsonia henryi.
| Locus | Primer sequences (5′–3′) | Repeat motif | Allele size range (bp) | Ta (°C) | BLASTX top hit description | E-value | GenBank accession no. |
| SH01 | F: TTTGACCCCGAAACAACAGC | (CAT)7 | 209–213 | 58 | — | MF503975 | |
| R: TGATACCGCTCAAGTCTCCC | |||||||
| SH02 | F: CATCACCTTCTGCTGGAACG | (TC)10 | 223–231 | 58 | Hypothetical protein EUGRSUZ_G03166 | 3E-40 | MF503976 |
| R: ACCCCGGAGCATATATCAGC | |||||||
| SH03 | F: CCACCTCGTTCTTCTCGTCT | (ATC)7 | 210–213 | 58 | Phospho-N-acetylmuramoyl-pentapeptide-transferase | 2E-176 | MF503977 |
| R: CCTGACGGTAAAGAGAAACGC | |||||||
| SH04 | F: GAGTCGGAGTCCCATTTTGC | (TTC)7 | 258–275 | 60 | NUDIX domain-containing protein | 3E-165 | MF503978 |
| R: GTCTTCGAACATGAGGCGTC | |||||||
| SH05 | F: TATGCTAGTGGTGGTGCTGT | (GCA)7 | 195–202 | 58 | — | MF503979 | |
| R: TAGTCGTCGGGCTCATCATC | |||||||
| SH06 | F: ATTGAAGGCGTTTGGATCCG | (GCC)7 | 148–158 | 58 | — | MF503980 | |
| R: TGGCTTCCCTCTCGTCTTTT | |||||||
| SH07 | F: TGACATGGAGGGTAGTGTGG | (ATG)7 | 183–186 | 58 | — | MF503981 | |
| R: TCACCTCTTCCATTGCCTTCT | |||||||
| SH08 | F: GAAGCTGGAGTTTGTGACGG | (GTT)8 | 214–225 | 58 | — | MF503982 | |
| R: CTTCGGGGCCTATAGTTGGT | |||||||
| SH09 | F: GGGGTGTTGTCCATTGATACAG | (ACC)7 | 232–240 | 58 | CBL-interacting protein kinase 07 | 0 | MF503983 |
| R: CCAGCAGTTGAAGTTCAGGAG | |||||||
| SH10 | F: AACCAAATCAGGCTCGCTTT | (AGC)7 | 225–239 | 59 | Pre-mRNA-splicing factor SYF1 | 0 | MF503984 |
| R: CCGCTGCCAGATGAAATTGA | |||||||
| SH11 | F: GGATTGCCATCATGCTGTTG | (TC)10 | 209–215 | 58 | Transmembrane protein 230 | 1E-61 | MF503985 |
| R: AGCAAATTTGGCCACTGGAG | |||||||
| SH12 | F: GGCATCCACAGTGTTGCTAG | (TC)10 | 154–156 | 58 | — | MF503986 | |
| R: ACTTCTGGGGCCATTTCCTT | |||||||
| SH13 | F: AAGGACGAGGATGAATGGGG | (GCG)7 | 265–268 | 56 | — | MF503987 | |
| R: CCCAATTCCCCTCGAGAAGT | |||||||
| SH14 | F: TCACCATCATCACCACCTTCA | (TTG)7 | 175 | 56 | ABC transporter G family member 5-like | 0 | MF510515 |
| R: AGGCTCATGGGTTTACAGCT | |||||||
| SH15 | F: AGCAAGAGGACCAACACTCT | (AAG)7 | 200 | 58 | — | MF510516 | |
| R: TGCTGCTTTTACTTCCCCTC | |||||||
| SH16 | F: CCAAGAGACCCCACCAACTA | (GCT)7 | 256 | 56 | — | MF510517 | |
| R: AGACGTTGCCTCAGTCTTGT | |||||||
| SH17 | F: TGGCTTCCAACCTCCTCAAA | (ACA)8 | 250 | 56 | — | MF510518 | |
| R: GGTGGGGTGGAAGAAGAGAG | |||||||
| SH18 | F: ACCCGCGATCATACTGACAA | (CTG)7 | 165 | 56 | DExH-box ATP-dependent RNA helicase | 3E-35 | MF510519 |
| R: GGTCCGTCATCACTTCTCCT | |||||||
| SH19 | F: GAGCAAACCCACAATCCAGA | (GAG)7 | 200 | 58 | — | MF510520 | |
| R: GCTGCCATGGTGAAGAAACA | |||||||
| SH20 | F: GGGTGGGGAGAATAGGGAAG | (CT)10 | 200 | 56 | NADP-dependent malic enzyme | 0 | MF510521 |
| R: AGAGGGAGAGAGGGTCACAA | |||||||
| SH21 | F: CCATATCCGCCGCCAATAAG | (GGT)7 | 275 | 58 | Receptor-like protein 1, putative isoform 2 | 2E-35 | MF510522 |
| R: GCTCAATTTGCTACCTTTGAAG |
Note: Ta = annealing temperature.