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RT-PCR showing expression of Gdnf and mRNA in oocytes and granulosa cells from antral follicles. Gdnf and Gfra1 bands shown were imaged after two rounds of PCR. Products from reactions with (+) and without (−) MMLV reverse transcriptase enzyme are shown. S2 was amplified as a constitutively expressed reference gene. Data are representative of a minimum of three different experiments.
