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. Author manuscript; available in PMC: 2017 Nov 27.
Published in final edited form as: J Neuroendocrinol. 2011 Dec;23(12):1204–1213. doi: 10.1111/j.1365-2826.2011.02211.x

Fig. 1.

Fig. 1

Immunocytochemical localisation of TRPM4 in the supraoptic nucleus (SON). Immunocytochemistry of TRPM4 staining in the SON using the ABC method with antibody dilution of 1 : 2500 in 40-µm coronal sections. (a) Localisation of TRPM4 immunoreactivity in the rat SON. Immunoreactivity was found in the cell bodies of magnocellular cells. (b) Absorption control in the adjacent section to that in (a) using the same antibody dilution. Almost all immunoreactivity was abolished when 20 µg of control peptide was added to 1 ml of antibody solution, confirming the specificity of the antibody to the immunogen designed to detect TRPM4. Scale bars = 50 µm.