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. Author manuscript; available in PMC: 2018 Sep 13.
Published in final edited form as: Sci Transl Med. 2017 Sep 13;9(407):eaad4000. doi: 10.1126/scitranslmed.aad4000

Fig. 3. Apelin induces endothelial FOXO1 inactivation and inhibits FABP4 expression.

Fig. 3

(A) FOXO1 phosphorylation in response to apelin stimulation (1 hour at the indicated concentrations) of HUVECs. (B) Time course of FOXO1 phosphorylation in HUVECs treated with apelin. (C) Subcellular localization of FOXO1 in response to apelin stimulation. Scale bar, 5 µm. (D) Apelin-induced FOXO1 phosphorylation in conjunction with inhibition of phosphoinositide 3-kinase by pretreatment with either Wortmannin or LY-294002. (E) Representative immunoblot of FABP4 in HUVECs in response to apelin knockdown (APLN si), FOXO1 knockdown (FOXO1 si), or both. (F) Representative immunoblot for FABP4 expression in isolated skeletal muscle ECs from the indicated mouse strains. *P < 0.05, **P < 0.01, and ††P < 0.01.