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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Vet Immunol Immunopathol. 2017 Oct 27;193-194:38–49. doi: 10.1016/j.vetimm.2017.10.004

Figure 1. Pathways related to the immune response are most significantly modulated in M. bovis-specific γδ T cells.

Figure 1

PBMCs were collected from calves (n=5) infected with virulent M. bovis and stimulated over-night with PPD-b or culture media alone. γδ T cells were isolated by magnetic separation and RNA was extracted and subjected to transcriptomics analysis. Differential gene expression values were calculated for each gene as the fold change of stimulated γδ T cells over mock treated γδ T cells for each animal. Genes commonly expressed between all five calves were subjected to a pathways analysis to reveal the most significant canonical pathways being represented by RNA sequencing results.